Journal: Neuroscience

Article Title: Linkage of non-receptor tyrosine kinase Fyn to mGlu5 receptors in striatal neurons in a depression model

doi: 10.1016/j.neuroscience.2020.02.048

Figure Lengend Snippet: (A) Effects of social isolation on pY416, Fyn, and Src levels in the CPu. (B) Effects of social isolation on pY416, Fyn, and Src levels in the NAc. Note that social isolation (SI) had no significant effect on phosphorylation and expression of Fyn and Src in the CPu (A) and NAc (B) as compared to control (Con) rats. Representative immunoblots are shown left to the quantified data. Data were statistically analyzed using Student’s t-test (n = 6 per group) with ‘n’ equal to the number of animals.

Article Snippet: A rabbit antibody against pY416 (RRID:AB_10013641; Cell Signaling) reacts with the SFK members when phosphorylated at a conserved activation residue, a pan Y416 site.

Techniques: Isolation, Expressing, Western Blot

Journal: Scientific Reports

Article Title: Bioactive glasses promote rapid pre-osteoblastic cell migration in contrast to hydroxyapatite, while carbonated apatite shows migration inhibiting properties

doi: 10.1038/s41598-023-47883-2

Figure Lengend Snippet: Migration of MC3T3-E1 pre-osteoblastic cells in response to different biomaterials and the expression levels of migration related proteins. ( a ) Number of migrated cells in the Boyden chamber assay at different time points. Results are shown as normalized to control within each time point. ( b – d ) Representative cropped images of the protein expression levels of FAK and SRC ( b ), pY576-FAK and pY529-SRC ( c ), and pY416-SRC ( d ) as detected by Western blot. Each figure ( b – d ) is from a separate membrane. Full membranes are available in Supplementary figure S4. ( e – i ) Quantitative analysis of protein bands is displayed for SRC ( e ), pY529-SRC ( f ), pY416-SRC ( g ), FAK ( h ), and pY576-FAK ( i ), where the control group was assigned as value of 1 within each blot. All data are presented as the mean ± standard deviation of three independent experiments. Parametric data was analyzed using unpaired t-test with Welch’s correction for unequal variances where applicable, and non-parametric data using Mann–Whitney test. Statistical significance is referred to as # p ≤ 0.05 between control and experimental group, and as *p ≤ 0.05, **p ≤ 0.01, and ***p ≤ 0.001 between indicated groups. HAP hydroxyapatite, CAP carbonated apatite, FAK focal adhesion kinase, BMP2 bone morphogenetic protein 2.

Article Snippet: Total protein (15 µg) was run on pre-cast gels (Bio-Rad, 4569033) and electro-transferred to polyvinylidene difluoride (PVDF) membranes, which were blocked with BSA in TBST (5% w/v), followed by incubation with primary antibodies for SRC (Invitrogen, PA5-17717, 1:1000), pY416-SRC (Invitrogen, PA5-97366, 1:1000, pY529-SRC (Invitrogen, 44-662G, 1:1000), FAK (Invitrogen, AHO0502, 1:100) and pY576-FAK (Invitrogen, PA5-104964, 1:750) diluted in TBST.

Techniques: Migration, Expressing, Boyden Chamber Assay, Western Blot, Membrane, Standard Deviation, MANN-WHITNEY